T regulative cells are involved in the peripheral tolerance mechanisms. From many old ages, the research has been traveling on to confirm the engagement of T regulative cells in systemic lupus erythematosus ( SLE ) in worlds. The of import subsets of T regulative cells are CD4+ and CD25+ T cells that act as the suppresser T cells in the which, it inhibits the “ activation and effecter map of autoreactive T cells ” . Skaguchi et Al foremost studied the lack of CD4+ and CD25+ subsets in the T-cells and the result of the trial was the development of autoimmune diseases ( T-cell mediated ) . The unequal supply of CD4+ and CD25+ T cells to the variety meats can take to the development of multiple autoimmune diseases. Along with the CD4+ and CD25+ molecules the look of the FoxP3 in the intracellular cells are of import as they help in modulating the T cells and suppressing the opportunities of developing the autoimmunity in worlds. CD45Ro+ is the other triping marker that can assist in bespeaking the function of T regular cells in SLE. SLE is autoimmune disease that besides links with the multiple defects in the individual enduring from this disease. From the old surveies, it has been hypothesized that the defect in the T regulative cells leads to the pathogenesis of the SLE. The assorted markers of T regulative cells can be tested which, can impart a manus in cognizing the defects in the T regulative cells in SLE or the tolerance mechanisms in the SLE.
Materials and Methods:
For the stuff in the trial, we can analyze the group of patients with SLE in an active and inactive province along with the group of the healthy controls to compare the consequences between them. The peripheral blood molecular cells ( PBMC ) are used to prove the degree of CD4+ and CD25+ T cells. The cell separation ( High gradient magnetic sorting ) method and flow cytometry can be valuable for the separation of CD4+ CD25- and CD4+ CD25+ . Other methods such as Real clip PCR or utilizing assay method or cytokine sensing can be impart a good manus in cognizing the function of T regulative cells in SLE.
To find that the T regulative cells are involved in SLE in homo we need to contrast the happening of CD4+ CD25+ , CD4+CD25+CD45+ and FoxP3 protein. We can besides correlate the consequences with the other diseases or production of autoantibodies. If the T regulative cells are involved in the development of SLE in worlds so the degrees of CD4+CD25+ should demo important difference between the SLE patient and the healthy control. If the experiment is carried out to prove the engagement of T regulative cells in SLE so the undermentioned consequences should be expected for the verification its engagement.
From the expected consequences of the CD4+CD25+ Treg, degree in SLE patient should be lower so the healthy control. The CD69 ( early activation marker ) and CD71 ( late activation marker ) were besides measured as for the comparing with the SLE patients and healthy controls. From the above figure 1, the degree of CD4+CD25+ Treg in the active SLE is lower when compared to the SLE inactive and healthy controls. The comparing of CD4+CD25+ Treg and CD4+CD25+CD45Ro+ T cells in SLE and HC. To do a differentiation in the T cells the FOXP3+ look is the most of import marker. At some point, it is difficult to distinguish the degrees of FOXP3+ in CD4+ from the SLE and HC. However, the degree of CD25+ cells in the SLE patients ( CD4+FoxP3+ ) is higher when compared with the healthy controls. From the survey, it can be hypothesized that the weakness in transition from FoxP3+CD25- to FoxP3+CD25+ leads to the decrease in the CD4+ CD25+ T cells in SLE patients. The above statement could be factual as the degree FoxP3+CD25- cells in SLA patients were higher. The
CD4+CD25+ T reg degree measured in SLE patients shows reduced degrees in comparing with HC and inactive SLE patients. Similarly, the FoxP3 protein degrees in the CD4+CD25high are higher in the healthy control, while the SLE patients show lower degree of FoxP3 protein degree.
Other of import factors that can lend more visible radiation on T regulative cells involved in development of SLE are the TNF, IL-12 and IL-2. Valencia. en al conducted a series of experiment which reveal that the damage in the T regulative cells in SLE patients. The ground that leads to the damage of T regulative cells is TNFRII. In the CD4+CD25+ subset the factor TNFRII degrees are higher ( That is the degree of TNF in the SLE patient is higher and the degree of CD4+CD25high is lower ) . This could be due to the complete production of TNFRII in SLE patient. Similarly, IL-2 besides contributes in suppression of CD4+CD25+ T cells by intervene with cell-cell contact.
The specificity and the pathogenesis of T regulative cells remains a enigma as the mechanisms of tissue hurt that arises in the SLE patients is still ill-defined. Although the trial consequences shows the defect in T regulative cells in SLE patients, the above trial consequences could non be counted as the collateral trial. Several grounds that encounter the standards that leads difficultly in verification of nipple consequences. For illustration, the CD25 molecule is a defeated marker because non all the CD4 + CD25+ T cells are T regulative cells, it is hard to distinguish between CD25+ from CD25- T cells in worlds, and even their findings do non show the suppressive activity. In worlds, the placed part of CD25+ in the CD4+CD25+ T cells has the high denseness and helps in operation of T regulative cells. Due to this ground, it is required to verify an imperfectness in the CD4+CD25high T regulative cells in SLE patients. Other possibility that might assist to unclutter the function of T regulative cells in SLE is the usage of corticoids or other drugs. For illustration, if the degree of CD4+CD25+ T cells/ FoxP3 additions by handling with corticoids to SLE patients. However, the potency of handling SLE patients therapeutically utilizing T regulative cells hold a great challenge in future.